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Wysłany: Śro 13:50, 09 Lut 2011
Temat postu: Tory Burch Shoes Prepared by electrodeposition ste
Prepared by electrodeposition step TiSBA / CHIT / AChE biosensor and its application
YAN Yong (Institute of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China) in recent years because of mesoporous molecular sieve with a large surface area, narrow pore size distribution and easy modulation, and good chemical stability and easy chemical modification, etc.,
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, as an enzyme immobilization of the vector has been used for biological sensor 【1]. But the natural zeolite pore size smaller, so applications are limited. Large and tunable pore size molecular sieve SBA. 15 solution to this problem that makes a breakthrough progress. Common methods of immobilized molecular sieve has a direct physical adsorption, embedding and chemical legitimate. But the prevalence of size selectivity, the enzyme is easy to fall off from the carrier and the enzyme activity and decreased the apparent lack of L21. Acetylcholinesterase (Acetyleholmesterase,
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, AChE) is present in vertebrates and insects, an important enzyme, which can rapidly hydrolyze the neurotransmitter acetylcholine excitatory synaptic transmission while maintaining the normal L3J,
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, the catalytic activity of organic phosphorus can be inhibited by pesticides, organophosphate insecticides are important targets. Using this feature can be made for the determination of organic phosphorus content of biological sensors. The preparation was chosen for the metal Ti-doped mesoporous TiSBA. 15, prepared by electrodeposition step in a TiSBC Ⅲ T / AChE modified electrode preparation is simple, controlled conditions, enzyme activity high. Will 30.0mgTiSBA. 15 and 0.3mLAChE (100mu) dispersed in 3.0mL0.5% CHI's HAe (pH5.0) solution, at 0. C, stirring 3h, by electrodeposition solution. In-3.0V constant potential, the deposition of 10min. I-I + in the cathodic reduction into H2, the cathode surface pH increases gradually. When pH> 6.3, the chitosan starting insoluble chitosan into TiSBA. 15, the AChE deposition to the electrode surface. CHIT / AChEAu prepared the same way. PBS solution at pH7.0, the bare electrode (Fig. 2a) and TiSBCmT / Au (Fig. 2b) no electrochemical oxidation and reduction peaks, AChESBCHIT / Au (Fig. 2d) appeared on the acetylcholinesterase in 980mV irreversible oxidation peak. Added to the solution of 0.2mol / L acetyl thiocholine (ATC1), AChE / TiSBA / CHIT / Au in the response current increases rapidly (Figure 20, ATC1 occur in the AChE catalytic oxidation,
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, hydrolysis products generated thio , and AChE / CHIT / Au (Fig. 2c) of only a very small current response. that TiSBA fixed for the AChE provides a good biocompatibility of the environment, metal Ti doping accelerated electron transfer rate of the electrode surface. will be placed AC1lE still SBA.15/Cm 20min carbaryl solution after soaking and found that significantly reduce the response current (Figure 2e), and with the increasing concentration of carbaryl decreased its current faster. Fig.2CVsofra) bareA (b) TiSBA / CI dish rI. / Aufd1ACIlE claw SBA sister T/Auin0.1mol/LpH7.0PBS; (c) TiSBA / CI-NT / Au; (f) AChE plant ISBA, CHIT, Auin0.1mo1/LpH7.0PBScontaining0.2mol/LArC1; (e) AChE Factory】 1iSBA, C Ⅲ Auimmersedin0.05I. tg/mLcarbarylsolutionfor20mil1. ScanRate: 50mV/sReferences1HarbnannM. Chem. Mater. ,2005,17:4577 - 45932HudsonS, Magngl Phys. Chem. B, 2005,109:19496 ~ 195063SilvanaA, Jean-Louis. M. Biomo1. Eng. ,2006,23:1-15 This article by the National Natural Science Foundation of China (No.20875039); Life Analytical Chemistry, Ministry of Education Research Fund (N0.KLACLS07004); Jiangsu University senior personnel Fund (No06JDG016) funded project 'E-mail : wanglam @ ujs. edu. en; Tel: +86511-8 ~ 169
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