HPLC determination of Scutellaria Ning ointment an

e707004304 · Wysłany: Sob 12:24, 19 Mar 2011

HPLC determination of ointment baicalensis Ning baicalin and hydrochloric acid content of Dyclonine

Key words: HPLC thymol SA CLC: R927.2 Document code: A Article ID :1672-7738 (2007) 10-0599-03SimultaneousdeterminationoftwocomponentsincomplexthymoleardropsbyHPLCHEYi-feng, LIUYi-zhao (theFirstPeople ' sHospitalofChangdeCity, Changde415003) ABSTRACT: OBJECTIVEToestablishamethodforthedeterminationofthym'olandsalicylicacidincomplexthymoleardrops. METHODSThecolumnwasshim-packVP-ODS (4.6 × 150mm, 5um), themobilephaseconsistedofmethanol - wa-ter-phosphoricacid (60:40:0.3), thedetectionwavelengthwas220nm, thecolumntemperaturewas30 RESULTSTherewasagoodlinearrelationshipwhenthecontentofsalicylicacidandthymolwasintherangeof0.12 ~ 0.28mg · m = a,[link widoczny dla zalogowanych], and0.06t0.14mg · mL-respectively. Theaveragerecoveriesofsalicylicacidandthymolwere101.2 (RSD = 0.57) and100.1 (RSD = 0.64), respectively. CONCLUSIONThemethodCanbeappliedtOthequalitycontrolofcomplexthymoleardrops. KEYWORDS: HPLC; thymol; salicylicacid ear drops containing compound thymol thymol, salicylic acid and other ingredients. For the treatment of ringworm better ear. In order to control its quality, relevant literature _l ~,[link widoczny dla zalogowanych], this study established a HPLC method, simultaneous determination of 2 ingredients preparation method can be used for the preparation of quality control. An instrument and reagent a Shimadzu LC 10A HPLC, Class ~ vp6.12 chromatography workstation,[link widoczny dla zalogowanych], thymol, salicylic acid reference substance (China Pharmaceutical and Biological Products), compound thymol eardrops ( hospital preparation room production, batch number: 070323,070518,070727), methanol (HPLC grade), phosphoric acid (AR), water (twice distilled). 2 Methods and Results 2.1 solution preparation 2.1.1 Preparation of reference substance stock solution; accurately weighed thymol 20.0mg, salicylic acid 40.0mg, with the home 100mL flask, add methanol and dissolved diluted to the mark, shake, reserve liquid as a reference substance use. 2.1.2 Preparation of test solution: precise amount of the test 5.0mL, home 100mL volumetric flask, diluted with methanol to the mark,[link widoczny dla zalogowanych], shake, then take this solution 5.0mL to 25mL volumetric flask, add methanol and diluted to the scale, shake spare 2.1.3 Preparation of blank solution; except thymol and salicylic acid, the amount of materials weighed prescription, preparation of the blank sample solution. Click 2.2 Chromatographic conditions and system suitability test column: Shim-packVP-ODS (4.6 × 150mm, 5m), mobile phase: methanol and water a phosphoric acid (60:40; 0.3), flow rate: 0. 8mL · min-, detection wavelength; 220am, the injection volume: lC'L. In this chromatographic conditions,[link widoczny dla zalogowanych], the measured retention time of salicylic acid and thymol were 5.3min and 17.6min, thymol theoretical plate number 10600. Ih'Jute $ A: blank matrix diagram