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Extensive atherosclerotic lesions than non-diabetic patients and severity of serious coronary heart disease incidence up to 26 Patients with chronic diarrhea, fecal indole isolated a negative pathogenic E. coli Chu Wenying Key words diarrhea; Escherichia coli / isolation & purification; fecal / microbiology; Indoles / metabolism in chronic diarrhea is Clinically,[link widoczny dla zalogowanych], persistent or recurrent diarrhea more than 2 months were. Pathogenic E. coli epidemic diarrhea in infants and children the main pathogen, is highly contagious, occurs in summer and autumn. Common clinical serotype 0 ,(),()() 2,[link widoczny dla zalogowanych], (). 28, serotype 0l27K63 rare. Indole-negative E. coli rare. Our hospital from the feces of adult patients with chronic diarrhea, an isolated indole-negative pathogenic E. coli oK. Serotypes, are reported below. Introduction cases of patients, male, aged 47, appeared in early April 2004, or painless diarrhea, abdominal pain 2, more than a month,[link widoczny dla zalogowanych], recurrent, delayed healing. Hospital stay in mid-June of Gastroenterology. Check the body temperature 37.7 ℃, thin appearance, the spirit of difference. Laboratory tests: WBC14.8 × 10 / I, N0.72, I0.22, M0.08. Stool: yellow, a small amount of mucus; microscopy: WBC0 ~ 2/HP; fecal bacterial culture isolated from pathogenic E. coli () K. Serotypes. The use of ceftazidime, ciprofloxacin, Chinese medicine, comprehensive treatment,[link widoczny dla zalogowanych], 10d after culture-negative fecal bacteria of clinical symptoms and discharged. Bacteriological identification 1. Training Features: according to conventional training, the stool was inoculated MacConkey, SS, blood agar, 35 ℃, after 24h culture l_】], 3 were grown on a medium round, smooth, moist, slightly convex, the edge of neat, medium-sized colonies, MacConkey and SS is pink, the Gram stain Gram-negative bacilli. 2. Biochemical characteristics: oxidase negative, positive lysine, ornithine, Author: 733000 People's Hospital of Gansu Province Wuwei City, reported cases of laboratory arginine, HS, indole, urease, phenylalanine, Citrus medica citrate acid were negative, fermented glucose, produce acid and gas, power positive, lactose, sucrose, maltose, xylose, arabinose were positive. OF is fermented, VP (+). 3. Serological diagnosis: to do with the slide agglutination test, and pathogenic E. coli OK polyvalent serum agglutination; and pathogenic E. coli diagnostic antisera unit () K. Agglutination; saline control was negative. According to the above characteristics, diagnosis of pathogenic E. coli () K serotypes. K-B susceptibility testing methods: antibiotic paper factory from Hangzhou Tianhe microbial agents produced by the WHO standard strains of Escherichia coli (ATCC25922) to do quality control. To ceftazidime, piperacillin, amikacin, ciprofloxacin and ceftriaxone are sensitive; of cotrimoxazole, gentamicin, ampicillin resistance. Conclusion indole-positive E. coli 98 as u, the strain is negative, we have repeated several times using different bacteria were negative. Biochemical response to this strain of pathogenic E. coli typical. Common infection in children in clinical, but in this case as adults. Therefore, routine stool cultures in the colonies as long as the typical, whether IMViC as one by one or a + + +, one by one, to be with the diagnosis of pathogenic E. coli polyvalent serum agglutination test done to rule out pathogenic Escherichia bacteria, to avoid missed.
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