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Dołączył: 21 Lut 2011
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PostWysłany: Pon 21:05, 07 Mar 2011  

Endostatin gene combined with ^ 32 P colloid growing cancer inhibition in rats


Gut. 21) 04; 53 (4) :561-7. [20084) 2-11 received sampan 2008-03-12 Revised] (ed. Hu Guoyi) DhHP eye drops on the experimental galactose cataract prevention and treatment of the text off the tree ZHAO Xiao-ming Li Wei Chen Xiaoguang Wang Licheng Wang Liping Yang Ming (Life Sciences, Jilin University University, Changchun 130021) (Abstract] Objective DhHP eye drops on the experimental galactose cataract in rats. Methods six sub-heme peptide as the main ingredient, preparation DhHP eye drops. Wistar rats with a half-replicate D. lactose model. the experiments were divided into 6 groups: control group, model control group, positive control group, model group (divided into high, medium and low dose group). In addition to the normal control group, each group D were injected half After cataract lactose to give the appropriate modeling of drug treatment. with slit lamp microscope, were observed moving objects dynamically shaped opacity of the occurrence time and circumstances,[link widoczny dla zalogowanych], and the first 4 weeks of the experiment were determined by crystal superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), glutathione (GSH) content. and the activity of serum SOD and MDA in the content. DhHP eye drops on experimental observations of galactose cataract in rats. Results DhHP rat cataract eye drops can significantly delay the occurrence of lens opacity time; reduce the degree of lens opacity; reduce the MDA content in cataractous lens; improve the body from the inside barrier materials like SOD, GSH-Px activity and GSH content. while reducing cataract in serum MDA, increased SOD activity. Conclusion DhHP eye drops with a better delay and reduce the role of galactose cataract. which may have a strong eye drops with DhHP antioxidant capacity. [Key words] sub-heme; D. galactose; cataract; antioxidant capacity [CLC] Q789 [Document code] A (Article ID] 1005-9202 (2009) 03-0286-- 03 mechanism of oxidative damage due to cataract has been recognized more and more ..., all kinds of anti-oxidants, substances have been used in experimental cataract prevention and treatment. However, until now all of these drugs did not reach satisfactory results . In recent years, antioxidant enzyme activity in the development of more and more attention. cytochrome C from the micro-enzyme hydrolysis of a MP 8, MP-9, MP 1l is a good peroxidase mimics , since the cataract in the lens model has proved its anti-cataract effect. but not easy to prepare and in vivo these compounds easily degradable, insoluble in water, characteristics also greatly limits its application. sub-heme peptide (DhHP- 6) has a high peroxidase activity and chemically induced rat lens cataract in rats. In this experiment, the application to DhHP as the main component of a 6 drops, its Effect on D galactose cataract in rats Crystal and serum antioxidant effects of its Fund Project: Changchun City to revitalize the old industrial base and the city of science and technology research, the hospital co-operation projects funded (O42GG221) 1 Breast Surgery, First Hospital of Jilin University, Corresponding author: Wang Liping (1967 a ),[link widoczny dla zalogowanych], female, Ph.D.,[link widoczny dla zalogowanych], professor, mainly engaged in drug discovery and design of the study. The first of the: Off Tree Man (1976 a), male, Ph.D., is principally engaged in the aging mechanism of anti-aging drugs. D galactose cataract mechanism, and an objective evaluation of its efficacy. 1 Materials and methods 1.1 Materials 1.1.1 Animal health 3W old Wistar rats weighing 70-90g, male and female, purchased from Changchun High-tech Research Center of Medical Experimental Animals, certificate number (Kyrgyzstan) in 2003 and 0004. conventional feed, free access to water. 1.1.2DhHP eye drops to research group developed six sub-heme peptide as the main component j, 100,50,25 ml preparation of three concentrations DhHP eye drops, after adjusting pH value and osmotic pressure-packing, and by co irradiation (radiation dose: 6kGy), sterilized for use. 1.1.3 topiramate Nuo Kexin sodium eye drops reagent were purchased from Wuhan Grand Pharmaceutical Group Co., Ltd.; U.S. Chardonnay (tropicamide) was purchased from Santen Pharmaceutical Co., Ltd. of Japan; D galactose Hanson for the Shanghai Chemical Reagent Co., Ltd.; superoxide dismutase (SOD), malondialdehyde (MDA ),[link widoczny dla zalogowanych], glutathione peroxidase (GSH-Px) and glutathione (GSH) kits were purchased from Nanjing Jiancheng Biological Engineering Institute. 1.1.4 UV-Vis instrument (Beckman Corporation USA ), YZ-5E-type slit lamp microscope (Suzhou Medical Apparatus),[link widoczny dla zalogowanych], low speed centrifugation


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