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ORANGE EKSTRAKLASA
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 Wysłany: Sob 15:44, 19 Mar 2011 |
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一株具有抗HBc及抗HBs双重反应性的抗HBV单克隆抗体细胞…
1张定丑乙型肝炎病毒见．妊定凤主编己堑肝兜盯发病机理与临床．重庆：重庆出版社．1992．1—232StandriugDN．MolecularanalysisofhepatitisBvirus．ProgLiverDist198616：1913祁进龙．部家育单克隆抗体及其临床应用f讲座)国外医学临床生物化学与检验分册．1988：9(3)244李方和，杨末亮，喻檀群．一种简便快速的YkAb筛选方法及其在单克隆抗体培养上清鉴定中的应用．单克隆抗体通讯，1993；9t2)：675王洪簿综述．单克隆抗体免疫交叉反应现象及其原围探讨．单克隆抗体通讯，1992,[link widoczny dla zalogowanych]；8(1)496FernandazPA，TernynchT．vrShn—munochemiealstud[es(farnurune!JolyreacnveIgG2bautoantibodyw；thRFaetivtyMolecu—larImmunology,[link widoczny dla zalogowanych]，989}26(65X9(L99311—09收稿)EstablishmentofaCellLinewithAnti-HBVMonoclonalAntibodywithTwo—foldImmunoreactivitiesagainstBothHBsAgandHBcAgLiFanghe，YangDongliang．YuZh[qunetalClinicalimmunologyResearchgnit．7~ngjlHospitat．TonMiMedical[fnlver．~．&y．~Vuha；,l430030AhstractInthepresentstudy，ahybridomaeeljline(E11)wasestablishedbyfusionnfmousemyelomacells(Sp2／0)withspleencellsfromBALB／emiceimmunizedwithttBVantigenpurifiedfromhumanplasmaandcontainingaplentyofHBVDaneparticles．ThemonoclonaIantibody(McAb)secretedbvE11eelIIinehastwofoldimmunoreactivitiesagainstbothHBsAgpolypeptides(HBeAg),[link widoczny dla zalogowanych]，HBVantigencontainingaplentyofDaneparti—des．HBVlightroundparticles(bothofthemcomefromhumanplasma)andHBsAgpositivehumanserumasproredbymultipIemethods．Thetiters0ftheMcAbascitesthatreactedwithabovefourHBVantigenswerebasicallysame。butitsimmunoreactivityagainstHBeAgwasstrongerthanthatagainstotherthreeantigens．ThespecificityoftheMcAbimmun0reactivitvwithabovementioaedHBVantigenshasbeenprovedbyblockingtestsandsubstitutiontests．Keywordsantibody，monoelonaI；hepatitisBvirus；antigenicspecificity；enzymelinkedimmunosorbentassay
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