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pH控制相转变分离-荧光免疫分析嗜水气单胞菌外膜蛋白
(生物医学标记示踪技术)．Beijing(北京)：PeoplesHealthPress(人民卫生出版社)，1995：16510ZhuQing-Zhi(朱庆枝)，YangHuang-Hao(杨黄浩)，LiDong-Hui(李东辉)，xuJin-Gou(许金钩)，ZhangYi(张毅),[link widoczny dla zalogowanych]，ZhangChang-Gong(张长弓)．Chem．ChineseUniversities(高等学校化学学报)，1999,[link widoczny dla zalogowanych]，20(4)：54J4～548DeterminationofOuterMembraneProteinofAeromonasHydrophilabypHControlledPhaseSeparationFluoroimmunoassayLINPeng，FENGJian-Jun,[link widoczny dla zalogowanych]，ZOUZhi-Hua，WANGShu-Hong，YANGQian，WANGYi-Lei(TheKeyLaboratoryofScienceandTechnologyforAquacultureandFoodSafety，FisheriesCollege，fimeiUniversity，Xiamen361021)AbstractAfluorescenceimmunoassaywasdevelopedforoutermembraneprotein(OMP)ofAeromonashydrophilausingapH—sensitivepolymerasacarrier．ThepH—sensitivepolymerwasconjugatedtotheanti—OMPantibodywithN—ethyl—N一(3-dimethylaminopropy1)carbodiimidehydrochloride(EDCI)．Inasandwichimmunoassay,[link widoczny dla zalogowanych]，OMPfirstreactedwiththeanti—OMPantibodyimmobilizedonpH—sensitivepolymerandthenfurtherreactedwithfluoresceinisothiocyanate(FITC)一labeledtheanti—OMPantibodyin37℃inhomogeneousformat．AfterchangingthepHtoseparatethepolymer—immunecomplexprecipitate，itwasre—dissolvedanddeterminedbyfluorescencemethod．ThecalibrationgraphforOMPwaslinearintherangeof0．4—30mg／Lwithadetectionlimitof31L．Themethodisrapid，sensitiveandsimple．Theimmobilizationefficiency，immunologicalreactionactivitiesofimmobilizedantibodywereimprovedasantibodywasimmobilizedviareac—tionofactivatedcarboxylicgroupbyEDC1withproteinaminogroup．KeywordsImmunoassay，pH—sensitivephase，separation，Aeromonashydrophila，outermembraneprotein(Received22November2007；accepted16January2008)
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