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yxfbbiedtj · Wysłany: Śro 4:30, 16 Mar 2011

Quantitative detection of hepatitis B HBV-DNA analysis and clinical significance of quantitative

And treatment plays an important role. But the qualitative PCR test to be open end, could easily lead to contamination of PCR products, causing false-positive results. Since 2000, Dezhou City,[link widoczny dla zalogowanych], Shandong Province People's Hospital of Zhongshan Medical University, using the provided PE5700 Tat Quantitative Genetic Analyzer,[link widoczny dla zalogowanych], of 500 cases of hepatitis B patients were PCR-HBV-DNA quantitative analysis for clinical diagnosis, drug selection and treatment determine the effect of providing a more reliable quantitative basis. Following results. 1.1 Case 1 object sources and methods: 500 samples were from hospital outpatient and inpatient. Of which 253 cases of hepatitis B carriers of the virus, acute hepatitis 42 cases, 181 cases of chronic hepatitis B, liver cirrhosis in 17 cases,[link widoczny dla zalogowanych], liver in 7 cases. Selected 181 cases of high, medium and low HBV-DNA replication with chronic active liver disease who 2b300 IFNa one million Methods: HBV-DNA quantitative PCR using real-time FQ - PCR, the initial template copy number as the common logarithm of the abscissa to the ordinate value of threshold cycle, with positive gradient standard standard curve to calculate the starting samples DNA content, testing equipment and reagents provided by the Tat Zhongshan Medical University. 500 cases of patients than did PCR-HBV-DNA were carried out outside the quantitative detection of serum markers of hepatitis B anti-HB-sAg - HBSHBeAg anti - HBe and anti - -HBc testing and liver function testing. 2 Results 2.1HBV-DNA quantitative detection and typing of hepatitis B in Table 1, the HBV carriers and chronic hepatitis B HBV-DNA quantitative asked significantly different, the results showed that carriers of hepatitis B immunization in the resistance by the state of the immune system does not recognize the virus,[link widoczny dla zalogowanych], so HBv-DNA replication activity. and chronic hepatitis B patients often inadequate antibody production, not the table 1HBV-DNA quantitative results between 20O3.3 with the amount of hepatitis B ■ 1l ... ~ a ~ ~ Kui LIdr / 'chHIdr /' | YlXH ● Table 2HBV-DNA quantitative results between serum markers of hepatitis B with HBV-DNA Table 3ALT quantitative relationship between the mean before treatment Table 4 Interferon and treatment of HBV-DNA quantitative HBV-DNA negative conversion rate after relations can pass the virus to wipe out the virus and immune system is in continuous state of war. acute hepatitis, liver cirrhosis, liver cancer and chronic hepatitis B IIBV-DNA showed significant quantitative differences were. acute hepatitis B immunization majority of normal, fast and thorough virus removal, cirrhosis, liver cancer patients in chronic active hepatitis after a long period, the virus integrated into the liver cells and free} Ⅱ 3V-DNA gradually reduced.} Ⅱ 3V-DNA quantitative results between serum markers of hepatitis B in Table 2. Table 2 shows the junior Yang IIBV-DNA quantitative means the highest.} Ⅱ 3V-DNA positive rate was 97%, with three positive difference is very significant. ALT and} Ⅱ 3V-DNA quantitative relationships shown in Table 3. As seen from Table 3, ALT decreased, BBV-DNA quantification mean is increased. HBv-DNA before interferon treatment and after treatment the mean quantitative} Ⅱ 3v-DNA negative conversion rates shown in Table 4, Table 4 showed low copy and moderate copy the effect of interferon therapy. The virus of low high degree of replication and viral replication between the effect of interferon is very significant. discuss Dezhou, Shandong Province People's Hospital on 500 cases of hepatitis B patients IIBV-DNA quantitative analysis showed that,[link widoczny dla zalogowanych], IIBV-DNA quantitative mean HBV carriers> Chronic hepatitis B> Acute Hepatitis B> liver> cancer, this result consistent with viral replication in hepatitis B patients regularity. BBV-DNA quantitative analysis lIBeAg positive, BBV-DNA mean the highest detection rate of 97%, while the anti - BBe BBV-DNA positive review a rate of only 18% (P <0.01) significant difference, the results confirm lIBeAg with IIBV-DNA detection rate is closely related. ALT and BBV-DNA quantitative analysis, ALT quantitative lower the higher the virus, ALT high indirectly reflects the immune status of the body. hepatitis B virus replication is the direct cause of chronic active hepatitis has been recognized. IIBV-DNA quantitative detection of the choice of antiviral drugs for clinical, efficacy evaluation and treatment of antiviral drugs to provide a reliable quantitative prediction based on . from interferon treatment and after treatment, quantitative BBV IIBV-DNA negative conversion rate analysis, low and moderate response to interferon a good copy. Currently the price of interferon still expensive, so the choice of cases should still be cautious, so as not to give the patient unnecessary financial burden. 20Q3.3