Electric pulse mediated cell fusion and blastocyst

e707004304

Electric pulse mediated cell fusion and blastocyst development of goldfish Ability

Therefore, the range of DNA variation between lc-l0c, but reflect the financial development of the embryonic cell nuclear transfer units is determined by several different DNA content of cells embedded platform body composition of the inference Leopard, these two experimental results are very similar. This shows that the integration of a blastocyst following nuclear transfer of chromosomes can lead exogenous human embryo. But the leaders had extensive chromosomal rearrangement and unequal distribution of the phenomenon. - From the past 0986) and present the results of these two experiments shows that nuclear transfer blastocysts by fusion of places you can move the mature unfertilized egg development, but the majority of embryos frozen at the blastocyst stage, but 14% of the embryos developed to the original brain, individual develop to juveniles. Most FNTB embryonic development stagnated. Speculate may be related with the uneven distribution of chromosomes (Fra-nkhause, 1934; He ~ / / feh; T963 ~, J.A.Moorl9 tin) o that the shift development to the dawn of the nuclear embryo may be a more balanced distribution of chromosomes o If in the future different DNA content of these isolated blastocyst cells,[link widoczny dla zalogowanych], screening the entire chromosome ploidy cells were cell nuclear transfer,[link widoczny dla zalogowanych], may be cultivated with different times of new varieties of fish sex chromosomes. ;! Foreign Summary (Abstract) ELEcTRILDMEDIATEDFUSIONOFASTOMF,[link widoczny dla zalogowanych], RESOFG0LANDITSDEVELOPMENTALPOTENTIAL. A GA0XIAOHONGLtGtrANGSANDoMIAOFENYANL/NOCoMamoa. ~ LoDBYU (InstiratedevelopmentalBiolog ~ AcademiuSimca,[link widoczny dla zalogowanych], Bei ~ ng) LxSOW ~ NtB Rights gNormalU more pcrsizh aldehyde Qin Bvusingsimpleelectrofu. sionme Hill od, tWOblgstomeresfromblastulastageofgoldfishwerefusedforthefirsttime. UnderoptimumconditFothefusionratemore, 'than95%. ndevelopmentalpotentialf0rthemlcleus cicada-like dcellwastestedthroughtransplantingiti Ⅱ tOanenuc1eatedunfertilizedegg: a Nucle ~ 'Illfuscellsweretransplhnted, ofwhich44developedintoblastulastage, sevenOOtofthe44furtherdevelopedintogastrulastageandoneofthe $ eveilfinal1develo ~ dintoalarva. DNAcoIltesl ~ ofthenucleifromeleyenblastulaederivedfromthenucleartransplantsweremeasuredafterHoechest33258stainingwithfluo-rescencemicrophotometry. inninecasestheDNAcontentsshowedfargreaterchangesthanthosefromenormalfertilizedblastulae. TheresultssuggestthatexogenotLschromosomemaybetransferredintoemb'ryosor ~ 'dultswiththemethodofe1ectrofusiofi. TtechrIiqHeopensupanewvenueforstudyingtheheredity0fsomaticcellsandgenetransferinfish. Keywords: Electrofusion, Nucleartransplantation,[link widoczny dla zalogowanych], DNAcontents,